Recombinant:Article Title: IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling response.
Article Snippet: Fetal bovine serum (FSD500, Excell Bio, China); Penicillin Streptomycin .. AR TIC LE IN PR ES S ARTICLE IN PRESS solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free IL-18 Protein, Human (His) (HY-P700108AF, Medchem Express, USA), IL-18-BP Protein, Mouse (HEK293, His) (HY-P75841 Medchem Express, USA); Recombinant Human IL-18-BPa Fc Chimera Protein, CF (#119-BP, R&D system, Minneapolis); L-PAM (HY-17575, Medchem Express, USA); Lipopolysaccharide binding protein (JN0536, Beijing Baiolaibo Technology, China); BCA protein assay kit (WB6501, Guangzhou Saiguo Biotech, China), ECL luminescent AB solution (P2100, NCM Biotech, China); Anti-Bcl-2 antibody [EPR17509] (ab182858, Abcam, UK), Anti-Bcl-XL antibody [E18] (ab32370, Abcam, UK), Anti-Caspase-3 antibody [EPR18297] (ab184787 Abcam, UK) Anti-Bax antibody [E63] (ab32503, Abcam, UK), Anti-Bad antibody [Y208] (ab32445 Abcam, UK), Anti-Cyclin D1 antibody [SP4] (ab16663, Abcam, UK), Antic-Myc antibody [Y69] - ChIP Grade (ab32072, Abcam, UK); Cox2 (D5H5) Rabbit Monoclonal Antibody (#12282, CST, USA), NF-kappaB p65 (D14E12) Rabbit Monoclonal Antibody (#8242, CST, USA); Anti-IL-6 [EPR23819-103] (ab290735, Abcam, UK); Anti-TNF alpha [EPR20972] (ab215188, Abcam, UK); beta-Actin (13E5) Rabbit Monoclonal Antibody (#4970, CST, USA); Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam, UK); CCK8 Cell Proliferation Detection Kit (BA00208, Bioss, China); Mouse IL-6 ELISA Kit (PI326, Beyotime, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China). ..
Binding Assay:Article Title: IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling response.
Article Snippet: Fetal bovine serum (FSD500, Excell Bio, China); Penicillin Streptomycin .. AR TIC LE IN PR ES S ARTICLE IN PRESS solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free IL-18 Protein, Human (His) (HY-P700108AF, Medchem Express, USA), IL-18-BP Protein, Mouse (HEK293, His) (HY-P75841 Medchem Express, USA); Recombinant Human IL-18-BPa Fc Chimera Protein, CF (#119-BP, R&D system, Minneapolis); L-PAM (HY-17575, Medchem Express, USA); Lipopolysaccharide binding protein (JN0536, Beijing Baiolaibo Technology, China); BCA protein assay kit (WB6501, Guangzhou Saiguo Biotech, China), ECL luminescent AB solution (P2100, NCM Biotech, China); Anti-Bcl-2 antibody [EPR17509] (ab182858, Abcam, UK), Anti-Bcl-XL antibody [E18] (ab32370, Abcam, UK), Anti-Caspase-3 antibody [EPR18297] (ab184787 Abcam, UK) Anti-Bax antibody [E63] (ab32503, Abcam, UK), Anti-Bad antibody [Y208] (ab32445 Abcam, UK), Anti-Cyclin D1 antibody [SP4] (ab16663, Abcam, UK), Antic-Myc antibody [Y69] - ChIP Grade (ab32072, Abcam, UK); Cox2 (D5H5) Rabbit Monoclonal Antibody (#12282, CST, USA), NF-kappaB p65 (D14E12) Rabbit Monoclonal Antibody (#8242, CST, USA); Anti-IL-6 [EPR23819-103] (ab290735, Abcam, UK); Anti-TNF alpha [EPR20972] (ab215188, Abcam, UK); beta-Actin (13E5) Rabbit Monoclonal Antibody (#4970, CST, USA); Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam, UK); CCK8 Cell Proliferation Detection Kit (BA00208, Bioss, China); Mouse IL-6 ELISA Kit (PI326, Beyotime, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China). ..
Bicinchoninic Acid Protein Assay:Article Title: IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling response.
Article Snippet: Fetal bovine serum (FSD500, Excell Bio, China); Penicillin Streptomycin .. AR TIC LE IN PR ES S ARTICLE IN PRESS solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free IL-18 Protein, Human (His) (HY-P700108AF, Medchem Express, USA), IL-18-BP Protein, Mouse (HEK293, His) (HY-P75841 Medchem Express, USA); Recombinant Human IL-18-BPa Fc Chimera Protein, CF (#119-BP, R&D system, Minneapolis); L-PAM (HY-17575, Medchem Express, USA); Lipopolysaccharide binding protein (JN0536, Beijing Baiolaibo Technology, China); BCA protein assay kit (WB6501, Guangzhou Saiguo Biotech, China), ECL luminescent AB solution (P2100, NCM Biotech, China); Anti-Bcl-2 antibody [EPR17509] (ab182858, Abcam, UK), Anti-Bcl-XL antibody [E18] (ab32370, Abcam, UK), Anti-Caspase-3 antibody [EPR18297] (ab184787 Abcam, UK) Anti-Bax antibody [E63] (ab32503, Abcam, UK), Anti-Bad antibody [Y208] (ab32445 Abcam, UK), Anti-Cyclin D1 antibody [SP4] (ab16663, Abcam, UK), Antic-Myc antibody [Y69] - ChIP Grade (ab32072, Abcam, UK); Cox2 (D5H5) Rabbit Monoclonal Antibody (#12282, CST, USA), NF-kappaB p65 (D14E12) Rabbit Monoclonal Antibody (#8242, CST, USA); Anti-IL-6 [EPR23819-103] (ab290735, Abcam, UK); Anti-TNF alpha [EPR20972] (ab215188, Abcam, UK); beta-Actin (13E5) Rabbit Monoclonal Antibody (#4970, CST, USA); Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam, UK); CCK8 Cell Proliferation Detection Kit (BA00208, Bioss, China); Mouse IL-6 ELISA Kit (PI326, Beyotime, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China). ..
Chromatin Immunoprecipitation:Article Title: IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling response.
Article Snippet: Fetal bovine serum (FSD500, Excell Bio, China); Penicillin Streptomycin .. AR TIC LE IN PR ES S ARTICLE IN PRESS solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free IL-18 Protein, Human (His) (HY-P700108AF, Medchem Express, USA), IL-18-BP Protein, Mouse (HEK293, His) (HY-P75841 Medchem Express, USA); Recombinant Human IL-18-BPa Fc Chimera Protein, CF (#119-BP, R&D system, Minneapolis); L-PAM (HY-17575, Medchem Express, USA); Lipopolysaccharide binding protein (JN0536, Beijing Baiolaibo Technology, China); BCA protein assay kit (WB6501, Guangzhou Saiguo Biotech, China), ECL luminescent AB solution (P2100, NCM Biotech, China); Anti-Bcl-2 antibody [EPR17509] (ab182858, Abcam, UK), Anti-Bcl-XL antibody [E18] (ab32370, Abcam, UK), Anti-Caspase-3 antibody [EPR18297] (ab184787 Abcam, UK) Anti-Bax antibody [E63] (ab32503, Abcam, UK), Anti-Bad antibody [Y208] (ab32445 Abcam, UK), Anti-Cyclin D1 antibody [SP4] (ab16663, Abcam, UK), Antic-Myc antibody [Y69] - ChIP Grade (ab32072, Abcam, UK); Cox2 (D5H5) Rabbit Monoclonal Antibody (#12282, CST, USA), NF-kappaB p65 (D14E12) Rabbit Monoclonal Antibody (#8242, CST, USA); Anti-IL-6 [EPR23819-103] (ab290735, Abcam, UK); Anti-TNF alpha [EPR20972] (ab215188, Abcam, UK); beta-Actin (13E5) Rabbit Monoclonal Antibody (#4970, CST, USA); Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam, UK); CCK8 Cell Proliferation Detection Kit (BA00208, Bioss, China); Mouse IL-6 ELISA Kit (PI326, Beyotime, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China). ..
Enzyme-linked Immunosorbent Assay:Article Title: IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling response.
Article Snippet: Fetal bovine serum (FSD500, Excell Bio, China); Penicillin Streptomycin .. AR TIC LE IN PR ES S ARTICLE IN PRESS solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free IL-18 Protein, Human (His) (HY-P700108AF, Medchem Express, USA), IL-18-BP Protein, Mouse (HEK293, His) (HY-P75841 Medchem Express, USA); Recombinant Human IL-18-BPa Fc Chimera Protein, CF (#119-BP, R&D system, Minneapolis); L-PAM (HY-17575, Medchem Express, USA); Lipopolysaccharide binding protein (JN0536, Beijing Baiolaibo Technology, China); BCA protein assay kit (WB6501, Guangzhou Saiguo Biotech, China), ECL luminescent AB solution (P2100, NCM Biotech, China); Anti-Bcl-2 antibody [EPR17509] (ab182858, Abcam, UK), Anti-Bcl-XL antibody [E18] (ab32370, Abcam, UK), Anti-Caspase-3 antibody [EPR18297] (ab184787 Abcam, UK) Anti-Bax antibody [E63] (ab32503, Abcam, UK), Anti-Bad antibody [Y208] (ab32445 Abcam, UK), Anti-Cyclin D1 antibody [SP4] (ab16663, Abcam, UK), Antic-Myc antibody [Y69] - ChIP Grade (ab32072, Abcam, UK); Cox2 (D5H5) Rabbit Monoclonal Antibody (#12282, CST, USA), NF-kappaB p65 (D14E12) Rabbit Monoclonal Antibody (#8242, CST, USA); Anti-IL-6 [EPR23819-103] (ab290735, Abcam, UK); Anti-TNF alpha [EPR20972] (ab215188, Abcam, UK); beta-Actin (13E5) Rabbit Monoclonal Antibody (#4970, CST, USA); Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam, UK); CCK8 Cell Proliferation Detection Kit (BA00208, Bioss, China); Mouse IL-6 ELISA Kit (PI326, Beyotime, China), Mouse TNF-α ELISA Kit (PT512, Beyotime, China). ..
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